Activity

Effect of Moringa oleifera, an age-old ingredient of Indian ayurvedic and traditional medicine, was tested for its effect on age related antioxidant activity in Wistar albino rats of three age groups (6, 12 and 18 months old). Aqueous extract of M. oleifera leaves (MOAE) was administered orally at a dosage of 200 mg/kg body weight for a period of 30 days. MOAE treatment showed significant reduction in lipid peroxidation and lipofuscin pigmentation along with elevated serotonin and antioxidant enzymes in the brains of treated groups of aged rats. LC-MS-MS analysis revealed blood brain barrier permeable secondary metabolites viz., 9,9-bianthracene, 4-Methoxycinnamic acid, Cinnamic acid, (E)-p-coumaric acid pyrogallol and ostruthin from the extract. 9,9-bianthracene and ostruthin showed better binding affinity to Keap-1 and SERT in silico. The present result suggests the protective efficacy of M oleifera against age related oxidative stress in brain.Objective. Limited models are available to predict work-relatedness of musculoskeletal disorders (MSDs) among semiconductor back-end workers. This study aims to develop a model to predict the MSDs development among back-end workers. Method. Potential MSD risk factors were extracted from 277 work compensation investigation reports conducted between 2011-2019. Binary logistic regression approach was used to determine significant predictors. Results. CM 4620 purchase Significant predictors (p  less then  0.05) include poor posture (odds ratio [OR] = 1.822; 95% confidence interval [CI] [1.261, 2.632]), forceful exertion (OR = 1.741; 95% CI [1.281, 2.367]), static posture (OR = 1.796; 95% CI [1.367, 2.378]), lifting and lowering (OR = 1.438; 95% CI [0.966, 1.880]), transferring (OR = 1.533; 95% CI [1.101, 2.136]), pushing and pulling (OR = 0.990; 95% CI [0.744, 1.317]), repairing machines (OR = 0.845; 95% CI 76 [0.616, 1.159]), preventive maintenance (OR = 1.061; 95% CI [0.765, 1.471]) and quality inspection (OR = 0.982; 95% CI [0.729, 1.322]). Confounding factors and employment duration played crucial roles in the model. Cross-validation of predictive model was 86.2%, while face validation among 30 experts was 7.9/10 (SD 1.9). Conclusion. The model allows practitioners to predict potential MSD cases among semiconductor back-end workers and proactively plan appropriate mitigation measures.People with aphasia demonstrate impaired production of bound inflectional morphemes, such as noun plurals and possession. They often show greater difficulty in marking possession versus plurality. Using a new tool for eliciting language, the Morphosyntactic Generation test, we assessed people with primary progressive aphasia and those in the acute and chronic phase following left hemisphere stroke. Clinical profiles were associated with different strengths and weaknesses in language production. Performance of the plural was stronger than possessive in group analyses. However, some individuals demonstrated the inverse pattern of performance. These participants provide counter-evidence to the theory that difficulty with marking possessives is purely the result of their greater cognitive-linguistic complexity and support a functional double dissociation between possessives and plurals. The deficits resulted from morphosyntactic impairment. Future work is needed to understand why plural and possessive markers were differently sensitive to neurological disorders of language.Objective. This study aimed to determine pulmonary functions and associated risk factors among school teachers. Methods. This cross-sectional ex-post facto study included 121 participants (20 males and 101 females). The setting for this study included 11 private schools and eight government schools. Participants were recruited using non-probability sampling techniques. Subjective data were collected using a self-administered asthma questionnaire. A spirometer was used for measuring pulmonary function values and data were analyzed using descriptive statistics and one-way analysis of variance for inferential statistics. The α level was set at 0.05. Results. Age, gender, duration of employment, type of school, school level taught, type of teaching board, working hours per day, previous occupation, usage of carpet and usage of rugs were significantly associated with the forced vital capacity prediction (p = 0.000, 0.010, 0.000, 0.032, 0.013, 0.000, 0.027, 0.000, 0.000 and 0.041, respectively). Also, the forced expiratory volume in 1 s and the peak expiratory flow were statistically different with alcohol consumption (p = 0.015) and place of residence (p = 0.004), respectively. Conclusion. Teachers using chalkboards are at increased risk of developing occupationally related pulmonary impairments. Hence, there is a need to shift from routine chalkboards to whiteboards.Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic OPLL. To date, the specific molecular pathogenesis of OPLL has not been clearly elucidated. In this study, bone marrow-derived mesenchymal stem cells obtained from healthy donors (HD-MSCs) and patients with OPLL (OPLL-MSCs) were cultured in osteogenic differentiation medium for 21 days. The osteogenic differentiation capacity was determined by alizarin red S (ARS) and alkaline phosphatase (ALP) assays. Gene expression levels of osteoblastic markers were measured by quantitative reverse transcription-polymerase chain reaction. Protein levels of related genes and the activation of related signaling pathways were measured by western blotting. LDN193189 was used to inhibit the Smad1/5/8 pathway, and small interfering RNA was used to regulate BMP2 expression. Our results showed that the OPLL-MSCs had stronger ARS staining and ALP activity and higher expression of RUNX2, Osterix, and OCN than the HD-MSCs. During osteogenic differentiation, the Smad1/5/8 pathway was overactivated in the OPLL-MSCs, and LDN193189 inhibition reversed the enhanced osteogenic ability of these cells. Besides, BMP2 was upregulated in the OPLL-MSCs. After BMP2 knockdown, the abnormal osteogenic differentiation of OPLL-MSCs was rescued. Thus, abnormal activation of the BMP2-Smad1/5/8 pathway induces enhanced osteogenic differentiation of OPLL-MSCs compared with HD-MSCs. These findings reveal a mechanism of pathological osteogenesis in OPLL and provide a new perspective on inhibiting pathological osteogenesis by regulating BMP2.